The following information has been sanctioned by the Department
of Environment and Labour.
Why should private water supplies be tested for bacteria
contamination?
The presence of bacteria such as coliform bacteria in drinking
water would indicate that the water is not safe for drinking
or cooking. People who drink water which has bacteria contamination
could become ill. Pathogenic (disease causing) organisms present
in the water are capable of causing gastro-intestinal disease.
What are coliform bacteria?
Coliforms are a group of bacteria widely distributed in nature.
They may be found in the soil and large numbers live in the
intestines of warm blooded animals, including humans. Natural
waters may become contaminated by animals (feces and urine),
by storm run-off, or by entry of raw and inadequately treated
sewage. As a group, coliforms are considered harmless, but their
presence in water is used as an indicator of other harmful bacteria.
Where should a water sample be collected?
The location for sampling should be the same location that you
would normally use to get your drinking water. In the majority
of facilities this would be the kitchen cold water tap.
Taking water samples for bacteria analysis directly from the
well is not recommended unless the proper sampling equipment
is used. Simply lowering a bottle into a well on a string or
using a dipper to collect the sample has a greater risk of becoming
contaminated with bacteria and showing incorrect results.
What is the correct procedure for collecting a water sample?
1. A proper container must be used to collect the water sample.
These containers can be obtained from any of the Department
of the Environment offices. The sample container must be kept
clean and free from contamination before and after collecting
the sample.
2. Remove any aerators, strainers, hose attachments, mixing
type faucets and purification devices from the taptourism_inspections_fr.aspcommon
spout, run hot water for two minutes to cleanse the faucet,
then switch to cold water for at least three minutes before
collecting the sample. Reduce the flow of water before taking
the sample to ensure no splashing occurs as the container is
filled.
3. While holding the container at the base, remove the cap with
the free hand, exercising care not to touch the inside of the
cap or the top of the bottle. The cap is to be held in one hand
during the entire sample collection operation; it is not to
be laid down.
4. The sample container must be filled to just above the "fill
line" marked on the bottle. The cap is carefully replaced.
5. The container and requisition form are to be labelled and
transported to the laboratory within 24 hours. The sample should
be kept cool until it arrives at the lab.
What do the water sample results mean?
Most laboratories do what is called a "presence/absence" determination
for coliform bacteria. The results of the test will indicate,
as the name implies, if coliform bacteria is present or absent
in the water sample. The test may be further expanded to indicate
both Total Coliform and Fecal Coliform organisms.
Total coliforms are the general group of coliform organisms
described above, whereas fecal coliform bacteria are normally
associated with sewage contamination. If results indicate that
the sample had "absence" for both the total and fecal coliform,
the water could be considered safe to drink from a bacteriological
point of view.
If the result showed the "presence" of either bacteria group,
the water should not be used for drinking until the source of
the bacteria contamination has been determined and corrective
action taken.
What assistance is available if coliform bacteria is found
in the water supply?
Assistance and advise is available from your local Department
of Environment office. Licensed well contractors can also provide
advice on well construction.
Crown copyright © 2009, Province of Nova Scotia, all rights reserved.
Page last updated 2009-07-30.